helicos true single molecule dna sequencing technology Search Results


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TopoGEN Inc super helical dna
Effects of PTE and RE <t>on</t> <t>Top1</t> relaxation activity. ( A ) Effects of PTE and RE at the different concentrations on Top1 relaxation activity. Lane 1, <t>DNA</t> marker; Lane 2, supercoiled plasmid DNA (pHOT-1, SC DNA); Lane 3, SC DNA+Top1; Lanes 4–9, SC DNA+Top1+RE/PTE; Lane 10, SC DNA+Top1+CPT. ( B ) Lane 1, SC DNA; Lane 2, SC DNA+ extract of CL 187 cells; Lanes 3–8, SC DNA+ extract of CL 187 cells treated with RE or PTE. Distribution of RE ( C ) and PTE ( D ) in CL 187 cells. Data are mean ± SD, n = 3.
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Siemens AG multidetector-row helical ct scanners somatom definition edge
Effects of PTE and RE <t>on</t> <t>Top1</t> relaxation activity. ( A ) Effects of PTE and RE at the different concentrations on Top1 relaxation activity. Lane 1, <t>DNA</t> marker; Lane 2, supercoiled plasmid DNA (pHOT-1, SC DNA); Lane 3, SC DNA+Top1; Lanes 4–9, SC DNA+Top1+RE/PTE; Lane 10, SC DNA+Top1+CPT. ( B ) Lane 1, SC DNA; Lane 2, SC DNA+ extract of CL 187 cells; Lanes 3–8, SC DNA+ extract of CL 187 cells treated with RE or PTE. Distribution of RE ( C ) and PTE ( D ) in CL 187 cells. Data are mean ± SD, n = 3.
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Helica Inc cdna library helica biosystems
Effects of PTE and RE <t>on</t> <t>Top1</t> relaxation activity. ( A ) Effects of PTE and RE at the different concentrations on Top1 relaxation activity. Lane 1, <t>DNA</t> marker; Lane 2, supercoiled plasmid DNA (pHOT-1, SC DNA); Lane 3, SC DNA+Top1; Lanes 4–9, SC DNA+Top1+RE/PTE; Lane 10, SC DNA+Top1+CPT. ( B ) Lane 1, SC DNA; Lane 2, SC DNA+ extract of CL 187 cells; Lanes 3–8, SC DNA+ extract of CL 187 cells treated with RE or PTE. Distribution of RE ( C ) and PTE ( D ) in CL 187 cells. Data are mean ± SD, n = 3.
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Helica Inc nucleic acid-dependent atpases helica~es
Effects of PTE and RE <t>on</t> <t>Top1</t> relaxation activity. ( A ) Effects of PTE and RE at the different concentrations on Top1 relaxation activity. Lane 1, <t>DNA</t> marker; Lane 2, supercoiled plasmid DNA (pHOT-1, SC DNA); Lane 3, SC DNA+Top1; Lanes 4–9, SC DNA+Top1+RE/PTE; Lane 10, SC DNA+Top1+CPT. ( B ) Lane 1, SC DNA; Lane 2, SC DNA+ extract of CL 187 cells; Lanes 3–8, SC DNA+ extract of CL 187 cells treated with RE or PTE. Distribution of RE ( C ) and PTE ( D ) in CL 187 cells. Data are mean ± SD, n = 3.
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Image Search Results


Journal: Cell Metabolism

Article Title: Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response

doi: 10.1016/j.cmet.2022.10.011

Figure Lengend Snippet:

Article Snippet: Antibodies used in this study: anti-phospho-p38 (Cell Signaling, mouse 9216, rabbit 4511S), anti-p38 (Cell Signaling, 9212, rabbit), anti-phospho-SAPK/JNK (Cell Signaling Technology, 9255, mouse), anti-SAPK/JNK (Cell Signaling, 9258, rabbit), anti-ZAK (Proteintech, 14945-1-AP, rabbit), anti-phospho-AMPK (Cell Signaling, 2535, rabbit), AMPK-alpha (Cell Signaling, 2532, rabbit), anti-p150 (BD biosciences, 610473, mouse), anti-Actin (Millipore, MAB1501, mouse), anti-α-Tubulin (Sigma-Aldrich, T9026, mouse), anti-HA-tag (Santa Cruz Biotechnology, sc-7392 HRP, mouse), anti-phospho-GCN2 (Abcam, ab75837, rabbit), anti-phospho-eIF2alpha (Cell Signaling, 3398, rabbit), anti-eIF2alpha (Cell Signaling, 9722, rabbit), anti-4EBP1 (Cell Signaling, 9644, rabbit), anti-Puromycin (Millipore, MABE343, mouse), anti-Ribosomal protein S10 (Abcam, ab151550, rabbit), anti-phospho-4E-BP1 (Cell Signaling, 2855, rabbit), anti-EDF1 (Abcam, ab174651, rabbit), anti-RPS2 (Bethyl, A303-794A, rabbit), anti-phospho-PERK (Cell Signaling, 3179, rabbit), anti-ASCC3 (Proteintech, 17627-1-AP, rabbit), anti- RPL19 (Novus, H00006143-M01, mouse), anti-phospho-p70 S6 kinase (Cell Signaling Technology, 9234, rabbit), anti-p70 S6 kinase (Cell Signaling Technology, 9202, rabbit).

Techniques: Recombinant, Reverse Transcription, Staining, Clone Assay, Mutagenesis, SYBR Green Assay, DNA Extraction, Enzyme-linked Immunosorbent Assay, RNA Sequencing, CRISPR, Knock-Out, Quantitative RT-PCR, Software

Effects of PTE and RE on Top1 relaxation activity. ( A ) Effects of PTE and RE at the different concentrations on Top1 relaxation activity. Lane 1, DNA marker; Lane 2, supercoiled plasmid DNA (pHOT-1, SC DNA); Lane 3, SC DNA+Top1; Lanes 4–9, SC DNA+Top1+RE/PTE; Lane 10, SC DNA+Top1+CPT. ( B ) Lane 1, SC DNA; Lane 2, SC DNA+ extract of CL 187 cells; Lanes 3–8, SC DNA+ extract of CL 187 cells treated with RE or PTE. Distribution of RE ( C ) and PTE ( D ) in CL 187 cells. Data are mean ± SD, n = 3.

Journal: Cancers

Article Title: Effect of Pterostilbene, a Natural Derivative of Resveratrol, in the Treatment of Colorectal Cancer through Top1/Tdp1-Mediated DNA Repair Pathway

doi: 10.3390/cancers13164002

Figure Lengend Snippet: Effects of PTE and RE on Top1 relaxation activity. ( A ) Effects of PTE and RE at the different concentrations on Top1 relaxation activity. Lane 1, DNA marker; Lane 2, supercoiled plasmid DNA (pHOT-1, SC DNA); Lane 3, SC DNA+Top1; Lanes 4–9, SC DNA+Top1+RE/PTE; Lane 10, SC DNA+Top1+CPT. ( B ) Lane 1, SC DNA; Lane 2, SC DNA+ extract of CL 187 cells; Lanes 3–8, SC DNA+ extract of CL 187 cells treated with RE or PTE. Distribution of RE ( C ) and PTE ( D ) in CL 187 cells. Data are mean ± SD, n = 3.

Article Snippet: Each sample included 2 units of Top1 (TopoGEN, Buena Vista, CO, USA) and 250 ng super helical DNA (pHOT-1, TopoGEN, Buena Vista, CO, USA).

Techniques: Activity Assay, Marker, Plasmid Preparation